Flash-Cooling of Xenon-Derivatized Myoglobin Crystals

• Mounted crystal in standard cryo-loop

• Xenon gas at 150 PSI for 20 min

• Vented to atmospheric pressure

• Flash-cooled in cold gas stream ~30 sec after depressurization

• Collected data set

• ~50% xenon occupancy

Asymmetric unit of the isomorphous difference Patterson, w = 0 Harker section for P6 Myoglobin. Largest peaks are ~18 ?.

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