Single Molecule Fluorescence Detection

The BBSRC has recently awarded a research grant to the VUV-IR group at CLRC Daresbury Laboratory to built a fluorescence microscope for single transmembrane receptor studies in living cells. The full text of the research application can be found here.
 

The microscope will be used to collect single pair fluorescence resonance energy transfer (smFRET) and  single molecule fluorescence polarisation (smFP) from a physiologically relevant group of receptors in a living cell. The SM data obtained from the new facility will provide information on, for example:

• The changes in axial and azimuthal angles of signalling cell-surface receptors

• The changes in the distance between receptor domains

Extracellular Domain Plasma Membrane Intracellular Domain

Workshop on SMFD at Daresbury Laboratory on the 20/11/02
 

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